| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.009491 |
| Chromosome: | chromosome 7 |
| Location: | 3008539 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333535 | (1 of 1) K04856 - voltage-dependent calcium channel T type alpha-1I (CACNA1I) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGGGATGTGGGCCGAGGCGTCGTGGAGCAGCAGGGCGAGCGGCGCCA |
| Internal bar code: | GGCGAGTGGCTATAAAGTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4664 |
| LEAP-Seq percent confirming: | 89.7959 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGCAGCATCAGGAAGTC |
| Suggested primer 2: | TGTGTGTGTGTGTGTTGGGA |