Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.009507 |
Chromosome: | chromosome 8 |
Location: | 3127963 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g376000 | (1 of 1) PTHR12906:SF0 - PROTEIN F44E7.9 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCATGTGCGCGCGTGAGGATGTGAGTGGTGACGTTCCAGTTGCGGG |
Internal bar code: | ACTCCCTACGGTAACGAGGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1483 |
LEAP-Seq percent confirming: | 93.1034 |
LEAP-Seq n confirming: | 27 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCATTGACCATTCGTACCGC |
Suggested primer 2: | AGCAAACACACATGCAGCAG |