Insertion junction: CLIP2.009510_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009510
Chromosome:chromosome 2
Location:7892119
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g145700 AOT1 Amino acid transporter; (1 of 2) K14207 - solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 2 (SLC38A2, SNAT2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACGGTTTGGCAAACAGCCGATTGCCGGTAGTGCCGCGTCGTGTGTGC
Internal bar code:TCACGATCGGATCGCGGAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2057
LEAP-Seq percent confirming:60.9375
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGCGTGTGATGGCAAAA
Suggested primer 2:GCAATACACATGGCGCGAAT