Insertion junction: CLIP2.009514_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.009514
Chromosome:chromosome 3
Location:5456421
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g184250 CAM9 Calmodulin-like protein; (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTCTACAACTGGTTGAACACGCCCCACTGCGACGCCGGTCACAAGGGT
Internal bar code:CAACACCAAAGCGGAACTTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1749
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCCGTATGTCTGTCTCTC
Suggested primer 2:CATGTTTTTGGTGTCGCGGT