Insertion junction: CLIP2.009530_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.009530
Chromosome:chromosome 13
Location:4662527
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g603950 CYN50,CYN14 Cyclophilin 50; (1 of 2) IPR002130//IPR029000 - Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain // Cyclophilin-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCATGCAACGTTACCCAACCACGTGTGTGCCCGTACAGGCCCGCACGCA
Internal bar code:TAAAAATTGTAACTGGGTCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1599
LEAP-Seq percent confirming:90.625
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACACATCGCGACTTCCA
Suggested primer 2:AATGCCTCTGAGTGTGCTCC