Insertion junction: CLIP2.009541_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009541
Chromosome:chromosome 12
Location:2851183
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g503200 (1 of 3) 3.4.24.13 - IgA-specific metalloendopeptidase / Immunoglobulin A1 proteinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACTGCAACCTCGCTCGCTCCTGCAGACTGCGGCTCTGGCGCCTCCTC
Internal bar code:AGCACTTTCTCAAAGACAGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3256
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTGACGACGGCCTCAAT
Suggested primer 2:GCGCATTGAACAGCTGCATA