| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.009552 |
| Chromosome: | chromosome 16 |
| Location: | 2818437 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662650 | NAT36 | N-acetyltransferase; (1 of 1) 2.3.1.82 - Aminoglycoside 6'-N-acetyltransferase / Kanamycin 6'-N-acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTCCCCACCCCAAGTGCCATCCCCCCTTTCTGTGGCGCCTCACCACC |
| Internal bar code: | GTCGAAAGTGGCTGGATCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACGGCAGTTGACTCCAA |
| Suggested primer 2: | CTGAGGAGGTTGCTTGGGAG |