| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.009576 |
| Chromosome: | chromosome 9 |
| Location: | 2622229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801018 | (1 of 1) PTHR11390//PTHR11390:SF21 - PROKARYOTIC DNA TOPOISOMERASE // DNA TOPOISOMERASE 3-ALPHA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCCTCACCCCATCCCCAGTTTCTCCTTGCAGCGCGACTGTTTCGGA |
| Internal bar code: | GTATGTCTCTCAGCCGGTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCGCGCATACAAAAGG |
| Suggested primer 2: | ACTCCCTCTCCCCTAAACCC |