| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.009576 |
| Chromosome: | chromosome 9 |
| Location: | 2622244 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801018 | (1 of 1) PTHR11390//PTHR11390:SF21 - PROKARYOTIC DNA TOPOISOMERASE // DNA TOPOISOMERASE 3-ALPHA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAAACTAAACCAAAACCAACGAAAACGAGGGGAGTGCAGGCAGAGGCG |
| Internal bar code: | GTATGTCTCTCAGCCGGTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCCTCTCCCCTAAACCC |
| Suggested primer 2: | GAGCTCGCGCATACAAAAGG |