| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009585 |
| Chromosome: | chromosome 4 |
| Location: | 1289519 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214690 | (1 of 1) PTHR22731:SF3 - RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1 | 5'UTR | |
| Cre04.g214700 | (1 of 4) PTHR12406//PTHR12406:SF27 - CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCATGTCAACCACGTGCATATGTTTCGACGCTAATAAAGTTGCATAG |
| Internal bar code: | ATCCTCAAAGGACGCAGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 70.3704 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCGGCACTTCACTTCAT |
| Suggested primer 2: | TGGTAATTACAACCCCGCCC |