Insertion junction: CLIP2.009589_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009589
Chromosome:chromosome 4
Location:1356781
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g214502 UGE,DIV113,UGE1,EZY4 (1 of 1) 5.1.3.2//5.1.3.5 - UDP-glucose 4-epimerase / Uridine diphospho-galactose-4-epimerase // UDP-arabinose 4-epimerase; UDP-D-glucose/UDP-D-galactose 4-epimerase 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGAGTGATGGGGTGCGAGACGGCGGGTGGTGAGTGCGTACAATGAGCG
Internal bar code:TATAGGAATTCGCGCACTGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:754
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCTTCGTGTTGTCTCCGT
Suggested primer 2:ACAACGAACGATGAGGCAGT