| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.009596 |
| Chromosome: | chromosome 3 |
| Location: | 3631279 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168300 | (1 of 1) 3.4.21.107//3.4.21.108 - Peptidase Do / Protease Do // HtrA2 peptidase / Serine proteinase OMI | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGCACGTCCTCCAACACCATCGTGGGTGCGGCCCACATGGTCGGCTT |
| Internal bar code: | CGCCTGTCGAGATATGTTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2280 |
| LEAP-Seq percent confirming: | 76.3636 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGGAGAGACTAGCCCA |
| Suggested primer 2: | AAGACACGAGGCGGAACATT |