Insertion junction: CLIP2.009599_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009599
Chromosome:chromosome 2
Location:8159281
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g144006 (1 of 17) PF13833 - EF-hand domain pair (EF-hand_8) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAGTAGGGAGAGGGGGGAGGGGGAGAAGGAGAGGGAGGAGGGGGAAGG
Internal bar code:TTTGATTCTGTGAGGAATGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2158
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGTAGGCTGCTGAGGTG
Suggested primer 2:ATTCAGGACGGTGTGGTGAC