| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009601 |
| Chromosome: | chromosome 3 |
| Location: | 5435458 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800346 | (1 of 10) PTHR11439//PTHR11439:SF185 - GAG-POL-RELATED RETROTRANSPOSON // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCTGTTGCAGCGCCGGTATATCGAGGAGCTGCTGCAGCGGCATCACA |
| Internal bar code: | AGTTGTCGCATCAGGGTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2527 |
| LEAP-Seq percent confirming: | 43.75 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCGTACAGGCTGAAGAGA |
| Suggested primer 2: | AGGGCCTTGACTTTGACGAG |