| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.009613 |
| Chromosome: | chromosome 12 |
| Location: | 5575633 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531000 | AMT1H,AMT8 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATGTGGTTGCACCGGCACACGACCAATGGAAGCCCGTGTCATGTGC |
| Internal bar code: | AAAATCACCATTGGCGGCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1397 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGTGCTGGTAGGATTGC |
| Suggested primer 2: | ACGACGACTCATTTGGCAGT |