Insertion junction: CLIP2.009635_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.009635
Chromosome:chromosome 2
Location:7745779
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g146650 TTL3,SSA11 (1 of 2) K16582 - tubulin polyglutamylase TTLL6/13 [EC:6.-.-.-] (TTLL6_13); Tubulin tyrosine ligase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGACTCTCGCTGTCGCCTGGAGATGGATGCTCTTGAGTGACGCAAGCCT
Internal bar code:GTTTTATAAAACTTTGTTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1818
LEAP-Seq percent confirming:67.2131
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCACGCCATAAGCTGCC
Suggested primer 2:AACACTCGTGGAGCACTGAG