Insertion junction: CLIP2.009644_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.009644
Chromosome:chromosome 8
Location:919073
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g361900 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGAAGGAGGGGGTGTTGAGGAGGGGTTGGGAGGGGTTGGGAGCGGGTG
Internal bar code:GGTAACTGATTCTTGGGGAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1061
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCACCCAACTCATGACTC
Suggested primer 2:ATTTCGTCCTGTCTGCGGTT