Insertion junction: CLIP2.009644_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.009644
Chromosome:chromosome 8
Location:919093
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g361900 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCCCCCCACCACCACCCACCTGCCAATACCCTGAGTTGTCAGACACC
Internal bar code:GGTAACTGATTCTTGGGGAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:679
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTCGTCCTGTCTGCGGTT
Suggested primer 2:CCCCACCCAACTCATGACTC