| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009648 |
| Chromosome: | chromosome 1 |
| Location: | 5161796 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036050 | MLH1 | DNA mismatch repair protein; (1 of 1) K08734 - DNA mismatch repair protein MLH1 (MLH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGACGCCACACTGCCCCGATGCGCGCTGCACATATACAACACTGGAAC |
| Internal bar code: | TTGGTATGTCCAACGGATTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTGGCTGGAGGATCTT |
| Suggested primer 2: | TCAACGCACCTGTAGTCCAC |