Insertion junction: CLIP2.009663_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009663
Chromosome:chromosome 12
Location:4458903
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g520200 CPL8,PPO5 (1 of 1) PTHR13343//PTHR13343:SF16 - CREG1 PROTEIN // SUBFAMILY NOT NAMED; Conserved in the Plant Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAACTCACATAAATGGTTTGGTTCTCGTCACCACTACCACCACCAAC
Internal bar code:ATTTTCTGCACTAGACCTATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2338
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAAACGTGCTGACTGGG
Suggested primer 2:CAGGACTCGGCTCGTGAAAT