| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.009669 |
| Chromosome: | chromosome 17 |
| Location: | 3632584 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725376 | (1 of 1) IPR000104//IPR001859//IPR009148 - Antifreeze protein, type I // Trypanosoma cruzi ribosomal protein P2-like // Streptococcal non-M secreted SibA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGAAGTGGGAATTTAGGAAGGTTTCGCGCGCGAGGGTCCGGTAGCA |
| Internal bar code: | GTCTTCGTCTAATCTTGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1279 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTAGTTCGGGTCTTTGCT |
| Suggested primer 2: | GTTGCATTCCCTGGAGAGCT |