Insertion junction: CLIP2.009687_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.009687
Chromosome:chromosome 16
Location:5207388
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g684600 SEC5 (1 of 1) K17637 - exocyst complex component 2 (EXOC2, SEC5); Component of the Exocyst Complex 3'UTR_intron
Cre16.g684650 (1 of 2) PTHR13748//PTHR13748:SF45 - COBW-RELATED // SUBFAMILY NOT NAMED; Putative metallochaperone intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCAGCAGCGAGTCCTACCCTGCTCACGGGCAGTCGCCCGGACTCTGC
Internal bar code:TACGTCAAGTTCCATTATCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1988
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTAGGTGGGTTGGAGGG
Suggested primer 2:CGTAATGGCACGCGTTACTG