Insertion junction: CLIP2.009711_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009711
Chromosome:chromosome 17
Location:3542716
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g724750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGCTTCCCCTGCCGTCCGCACAGAATTTCCGGCGGATGATGATGCCG
Internal bar code:CGTCTAAAATAGGCTGGTTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2501
LEAP-Seq percent confirming:89.5833
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGCAGAAGGTTGTGGCC
Suggested primer 2:ACTCTGCTACAAACGCGTGA