Insertion junction: CLIP2.009724_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009724
Chromosome:chromosome 3
Location:8972622
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g203793 GT90-35,GT90F35 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 35 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGGAAGTAAGGCTGGGAACAAGTCGCGTCGCTCAAACCTCCAACGAC
Internal bar code:TTACAGATCATAGCGTCGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1770
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:TCGGAGTAGGCGTTTGCTTT
Suggested primer 2:TCCATGTCTCCAAACAGGCC