Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.009741 |
Chromosome: | chromosome 7 |
Location: | 1952746 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g325754 | (1 of 3) 2.7.11.25//2.7.12.1 - Mitogen-activated protein kinase kinase kinase / MLTK // Dual-specificity kinase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGGCGCCCCCCCCGGCCCGCCTCACCCGAAGTCTGCCAGCTTGGCT |
Internal bar code: | CCGAGAAGCAACAGCTGAAACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 662 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 7 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGTCCAGGTATGACTAGGC |
Suggested primer 2: | AGCAAGGGAGTAGCAAGCAG |