| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.009749 |
| Chromosome: | chromosome 16 |
| Location: | 5147303 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685300 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGGGGCGTTCGCAGGCAAATGGCGCGGGACATTGTTGCCGCGCCCA |
| Internal bar code: | CGAAAAACAGTTTAATCTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1322 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTTTGCTGGGATCCTCT |
| Suggested primer 2: | TAGTCAAGAGCCTTGCGCAA |