| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.009769 |
| Chromosome: | chromosome 16 |
| Location: | 4866583 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687117 | (1 of 2) PF14249 - Tocopherol cyclase (Tocopherol_cycl) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGCGCAGAGCGCACGTGCATTGGAGCCCCGCCGCCGTTCAGACAC |
| Internal bar code: | ATGCTAGAATTTGGGAATGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2893 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGGAAGTCCCAACGCAG |
| Suggested primer 2: | AAGCTGACCTGCCCATCTTC |