Insertion junction: CLIP2.009820_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009820
Chromosome:chromosome 13
Location:3365802
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g586650 FXL1 FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACCCAACTGCGTCTGCTCACACAAATCCCCCTGACCCACCTTCACGG
Internal bar code:CTTGCACCTACCCTACACTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4816
LEAP-Seq percent confirming:95.5224
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:AAACGCAACCCACATCGTTG
Suggested primer 2:AATCTTGAGGCGGGTGACTG