Insertion junction: CLIP2.009827_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009827
Chromosome:chromosome 16
Location:4175219
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCACCCCATCACGACGACGCCACCCCTCCACACCAATCCACCACCTTC
Internal bar code:GCCCTTTTCCCAACGGTGCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1053
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGCGTGTGTGAAGGGTC
Suggested primer 2:CGTCACGCATGCAACCATAG