| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.009828 |
| Chromosome: | chromosome 12 |
| Location: | 5273622 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527950 | (1 of 1) 2.4.1.227 - Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase / Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase; Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAGCATTGGAGAGCACAGGGCACGCCGCCTCTCCCAAAACGAGCACA |
| Internal bar code: | TATATTTGTAGGTTAGGCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1575 |
| LEAP-Seq percent confirming: | 95.7447 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCATCTGAGCTCAACA |
| Suggested primer 2: | TTGGGCTCATACACCAGCAG |