| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.009831 |
| Chromosome: | chromosome 11 |
| Location: | 2692750 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095071 | (1 of 2) K01148 - poly(A)-specific ribonuclease (PARN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAAGCCTGCACCCGCACGAACAAGCAAGAAACTGTCGCTGTTAATGC |
| Internal bar code: | ATGATCGGTCTCTAGTAGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2222 |
| LEAP-Seq percent confirming: | 68.3761 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 117 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCGTTGTGTCCGTCCAG |
| Suggested primer 2: | AAGCTGTTGCCTCCTGAGAC |