Insertion junction: CLIP2.009832_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.009832
Chromosome:chromosome 1
Location:5289285
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g037150 CLV6 (1 of 1) PTHR11689//PTHR11689:SF14 - CHLORIDE CHANNEL // CHLORIDE CHANNEL PROTEIN 2; ion channel/ voltage-gated chloride channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCCAGCGGCGAGAAGTTGTCGTCCACCACCACCGCCGCACTGCTGCC
Internal bar code:TTTTTGGAAGGTTTTTCTGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:385
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGGAGTGGGGATAGGGT
Suggested primer 2:GTTTACCCCAAACCACACGC