Insertion junction: CLIP2.009845_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.009845
Chromosome:chromosome 3
Location:1864648
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g154600 (1 of 2) PTHR35514:SF1 - THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGTTGTCTTACACACGTCTCCCGCACAACCACCATGCCGCGTCCATA
Internal bar code:ACTGCGCGTATTACGTATTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTTTCCTCCGTGGTAA
Suggested primer 2:AAGCACAACCAATCCAACGC