| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.009886 |
| Chromosome: | chromosome 3 |
| Location: | 2098741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156450 | 5'UTR | ||
| Cre03.g156500 | (1 of 1) IPR000104//IPR016084 - Antifreeze protein, type I // Haem oxygenase-like, multi-helical | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGACAAGCTTTAGTTCGGAGCAATCATTGCTAGCACGCGTGGCCTCAT |
| Internal bar code: | ACGATACGGATGGCATTACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2154 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATTGTTTCCCAAACCCC |
| Suggested primer 2: | CCTCGAACTCGACGATCAGG |