Insertion junction: CLIP2.009887_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.009887
Chromosome:chromosome 3
Location:201128
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144164 RSE2,RSEP2 (1 of 1) PTHR22939:SF79 - PEPTIDASE M50 FAMILY PROTEIN; Intramembrane metalloprotease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCGGATCATGTGGTTCTGCCATCTGCTCCCCCGCTGCGCTTTGAGGA
Internal bar code:AATTGGCGTCTAAGTTCCACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:457
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACTTCCAAATGGCCGGTCC
Suggested primer 2:TTGGTCGTCACAGGCTTCTC