| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009889 |
| Chromosome: | chromosome 9 |
| Location: | 5277591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407200 | POD1 | Putative all-trans-retinol 13%252C14-reductase; (1 of 3) 5.2.1.13 - Prolycopene isomerase / CRTISO | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGCGAGCCTTCCTGGACCTGGAGGTGTGTGTGTGTGGGGGGGGGGC |
| Internal bar code: | AGCGTCAGACTGTGTATCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGATAGCGAGAGGGTGAC |
| Suggested primer 2: | AGGGGCGGGAATGTTTTAGG |