Insertion junction: CLIP2.009897_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.009897
Chromosome:chromosome 2
Location:4404814
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g105750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCGTTGATCGCGCCGGGCAGGTGCGGAAACGTGCAGACCATGTCTTC
Internal bar code:AGTTATGGGGAGGTAACGATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1947
LEAP-Seq percent confirming:97.8723
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGTCAAAAACAGTGCGCA
Suggested primer 2:AGTCTTTTCGCAGGCCTGTT