Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.009899 |
Chromosome: | chromosome 3 |
Location: | 3861467 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g170400 | (1 of 1) PF12579 - Protein of unknown function (DUF3755) (DUF3755) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCGTGCTTTTGTCCACCGAATTCTGTGACGAAGTGTTTGCTACTGGT |
Internal bar code: | GATAGGTGTCGGGTGTTCAAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1753 |
LEAP-Seq percent confirming: | 98.4375 |
LEAP-Seq n confirming: | 63 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACATCACGGGGGCTTTGAAT |
Suggested primer 2: | GTCCCGATAGCGCATGAGAA |