| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.009922 |
| Chromosome: | chromosome 10 |
| Location: | 1601733 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429150 | PRT1 | Anthranilate phosphoribosyltransferase; (1 of 1) 2.4.2.18 - Anthranilate phosphoribosyltransferase / Phosphoribosyl-anthranilate pyrophosphorylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAGCCCAGCCCAGTCCGCCCATTCGCGTTGCCAGCACCGCAACCACC |
| Internal bar code: | AATTAGGTCGCGTGTAGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2486 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGCTTCCCTGCAACAC |
| Suggested primer 2: | AGCTAGTTCAGTCGGCGAAC |