Insertion junction: CLIP2.009925_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009925
Chromosome:chromosome 15
Location:896975
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g640150 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGGAAGGAAGGGAACAATCTTCATAATGTTTACCGCAACACATAGTGC
Internal bar code:GCAAAACAAGTTGTTGTGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1131
LEAP-Seq percent confirming:84.6154
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACGCCATGCAACACTTG
Suggested primer 2:CTGAATCTCCTGCAGCTGCT