Insertion junction: CLIP2.009934_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.009934
Chromosome:chromosome 14
Location:3663635
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g631150 CEP14 (1 of 1) 2.4.2.26//2.7.11.1 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; Cysteine endopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTGCGCTGAGGCAGGCGTCACCGACGCCACCTGCACAGCCGTGTCGG
Internal bar code:TATGGGGACATATGATAATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1388
LEAP-Seq percent confirming:75.9259
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCAAAACTTCGTGGGACC
Suggested primer 2:GATGTCGTCTGCGCCAAATC