Insertion junction: CLIP2.009953_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.009953
Chromosome:chromosome 11
Location:362395
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467577 (1 of 2) PF00319 - SRF-type transcription factor (DNA-binding and dimerisation domain) (SRF-TF) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGTCCAGCACATCCATGGGCGCCAGGCCGCGTAGGCTGCTGTCCAGCG
Internal bar code:GTAGGTGGTCTCTAACTCAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1347
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCAAGCCGCTCTCCATCA
Suggested primer 2:ACCTCCAACCCATCCAACAC