Insertion junction: CLIP2.009978_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.009978
Chromosome:chromosome 9
Location:3226943
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394732 AZI1,MOT3,CEP131 Centrosomal Protein 131; (1 of 1) K16540 - 5-azacytidine-induced protein 1 (AZI1, CEP131) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCTCCCGGATGGCCGCCACCCGCTCCGCCACCTCCTTGCGGGCGCGC
Internal bar code:GCGAGTGGATACATTTAACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1912
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATTAGCAAACCGTGGGA
Suggested primer 2:CGTCCATCACACAGTCCACA