Insertion junction: CLIP2.009978_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.009978
Chromosome:chromosome 9
Location:3226945
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394732 AZI1,MOT3,CEP131 Centrosomal Protein 131; (1 of 1) K16540 - 5-azacytidine-induced protein 1 (AZI1, CEP131) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCTGGAGGAGCGAAACAGCGAGATACAGGTGGGCAGCGCGGCACTGG
Internal bar code:GCGAGTGGATACATTTAACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1109
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCCATCACACAGTCCACA
Suggested primer 2:GGGATTAGCAAACCGTGGGA