Insertion junction: CLIP2.010004_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010004
Chromosome:chromosome 10
Location:1935541
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g431800 ARS5 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAAAATACTTCTCAGAAGCTGTCTGCGAAGTCGATGAACTATTGGCAC
Internal bar code:GAAACAAGGGGAAGGTCCCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:69
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTATACATTTGCAGCGC
Suggested primer 2:GGTCGCCGATGTATTTGTGC