| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.010012 |
| Chromosome: | chromosome 14 |
| Location: | 1219433 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615800 | (1 of 3) PTHR31563:SF1 - ION CHANNEL POLLUX-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTAGCACTCCTCCCTCCCGATGGCCTGCCTCCCCGCTCCCCGCACCTCC |
| Internal bar code: | CTGACTCTCATTCGGTGTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 64.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTGCAGCACTCCCTCTC |
| Suggested primer 2: | GGTCTATAACTCCCGTGCGG |