| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010014 |
| Chromosome: | chromosome 1 |
| Location: | 537469 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003300 | (1 of 2) PTHR30483 - LEUCINE-SPECIFIC-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCCATCATCCTGCCCGTGCCTGCCTGCCCACCACGCACCCTCAAAAC |
| Internal bar code: | AAAGGGGGAGAGTAGTATCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2028 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATGATGTCGCCGTTCTC |
| Suggested primer 2: | CCCAAGCTCCCCATTGTCAT |