| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.010016 |
| Chromosome: | chromosome 13 |
| Location: | 1917133 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575950 | HIRA1,HIR1 | (1 of 1) K11293 - protein HIRA/HIR1 (HIRA, HIR1); Histone transcription regulator | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAAGCAACACGCAAATCGCCACACAGGCGGTGCTGGAGCACAACCTGG |
| Internal bar code: | TCGTGCTGACTGTGTCCAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2572 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATTTCTTCCCCTCCCAC |
| Suggested primer 2: | GCGCATGCAGTTACCGTATG |