| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.010019 |
| Chromosome: | chromosome 11 |
| Location: | 1841759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467400 | (1 of 3) IPR000104//IPR001859 - Antifreeze protein, type I // Trypanosoma cruzi ribosomal protein P2-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACGGCAACACGTGCCTGCGGACCACGAAAGTGCCGGTTACGCACGA |
| Internal bar code: | GTACCCTCCATGTGCGTAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2586 |
| LEAP-Seq percent confirming: | 93.0556 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCCAGGTATGCTCCTAAG |
| Suggested primer 2: | ATCCCACTCCATCCCCATCA |