| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010042 |
| Chromosome: | chromosome 12 |
| Location: | 5333844 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528614 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCGTTAGTGCTGCCGCCATTGGTCCCGTCAGGCCGAATGGCTGACGC |
| Internal bar code: | AAGAATTTGTGGTTGAAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3112 |
| LEAP-Seq percent confirming: | 84.0708 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 113 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTGCAGGCTCCTTTGTA |
| Suggested primer 2: | ACTTTATCACCCGCGCTTGA |